EGR2 antibody (Internal Region)

Catalog No. ABIN334389

This anti-EGR2 antibody is a Goat Polyclonal antibody detecting EGR2 in WB and ELISA. Suitable for Human.

Quick Overview for EGR2 antibody (Internal Region) (ABIN334389)

Target: EGR2 (Early Growth Response 2 (EGR2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EGR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-EGR2 Antibody

Binding Specificity: Internal Region

Purpose: EGR2

Sequence: HGTAGPDRKP FPC

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence HGTAGPDRKPFPC, from the internal region of the protein sequence according to NP_000390.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55 kDa band observed in lysates of cell line HepG2 (calculated MW of 50.3 kDa according to NP_000390.2). Recommended concentration: 2-6 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EGR2

Target: EGR2 (Early Growth Response 2 (EGR2))

Alternative Name: EGR2

Background: EGR2, early growth response 2 (Krox-20 homolog, Drosophila) , CMT1D, CMT4E, DKFZp686J1957, FLJ14547, KROX20, KROX-20, Drosophila, homolog (early growth response-2), Krox-20 homolog, OTTHUMP00000062133 , early growth response 2 protein

Gene ID: 1959, 13654, 114090

NCBI Accession: NP_000390