GABRB3 antibody (Internal Region)

Catalog No. ABIN334404

This anti-GABRB3 antibody is a Goat Polyclonal antibody detecting GABRB3 in IHC and ELISA. Suitable for Human.

Quick Overview for GABRB3 antibody (Internal Region) (ABIN334404)

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GABRB3 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-GABRB3 Antibody

Binding Specificity: Internal Region

Purpose: GABRB3

Sequence: EKTAKAKNDR SK

Specificity: This antibody is expected to recognize both reported isoforms (NP_000805.1, NP_068712.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKTAKAKNDRSK, from the internal region of the protein sequence according to NP_000805.1, NP_068712.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Cortex shows patchy staining near or at the neuronal cell membrane.. Recommended concentration, 2-4 μg/mL.

Western Blot: Preliminary experiments in Human Brain (Cerebral Cortex, Frontal Cortex and Cerebellum) lysates gave no specific signal but low background (at antibody concentration up to 1 μg/mL). We would appreciate any feedback from people in the field -

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GABRB3

Target: GABRB3 (gamma-aminobutyric Acid (GABA) A Receptor, beta 3 (GABRB3))

Alternative Name: GABRB3

Background: GABRB3, gamma-aminobutyric acid (GABA) A receptor, beta 3, MGC9051, GABA(A) receptor beta-3 subunit, GABA-A receptor beta-3 subunit, GABA-alpha receptor beta-2 subunit, GABAA receptor beta-3 subunit

Gene ID: 2562, 14402, 24922

NCBI Accession: NP_000805, NP_068712

Pathways: Sensory Perception of Sound