HYAL1 antibody (Internal Region)

Catalog No. ABIN334421

The Goat Polyclonal anti-HYAL1 antibody has been validated for IHC, ELISA, IF and FACS. It is suitable to detect HYAL1 in samples from Human.

Quick Overview for HYAL1 antibody (Internal Region) (ABIN334421)

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HYAL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-HYAL1 Antibody

Binding Specificity: Internal Region

Purpose: Hyaluronidase 1

Sequence: ENTRTKESCQ AIKEY

Specificity: This antibody is expected to recognise isoform 1 (NP_009296.1), isoform 3 (NP_695015.1) and isoform 5 (NP_695017.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence ENTRTKESCQAIKEY, from the internal region of the protein sequence according to NP_009296.1, NP_695014.1, NP_695015.1, NP_695017.1,.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Liver. Recommended concentration: 5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration:

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HYAL1

Target: HYAL1 (Hyaluronidase-1 (HYAL1))

Alternative Name: HYAL1

Background: Hyaluronoglucosaminidase 1 , HYAL-1, LUCA1, MGC45987, NAT6, hyaluronidase 1, plasma hyaluronidase, tumor suppressor LUCA-1

Gene ID: 3373

NCBI Accession: NP_009296, NP_695014, NP_695015, NP_695017

Pathways: Glycosaminoglycan Metabolic Process