MBD2 antibody (Internal Region)

Catalog No. ABIN334432

This Goat Polyclonal antibody specifically detects MBD2 in WB, ELISA and IHC. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for MBD2 antibody (Internal Region) (ABIN334432)

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This MBD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-MBD2 Antibody

Binding Specificity: Internal Region

Purpose: MBD2 (isoform 1)

Sequence: RNDPLNQNKG KPDLN

Specificity: This antibody is expected to resognize isoform 1 (NP_003918.1) only.

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RNDPLNQNKGKPDLN, from the internal region of the protein sequence according to NP_003918.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Colon, Adrenal Gland and Skin. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 48-50 kDa band observed in lysates of cell line Jurkat (calculated MW of 43.3 kDa according to NP_003918.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Tan et al, Mol Cell Bio

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-MBD2 antibody (ABIN334432)

Bartels, Spruijt, Brinkman, Jansen, Vermeulen, Stunnenberg: "A SILAC-based screen for Methyl-CpG binding proteins identifies RBP-J as a DNA methylation and sequence-specific binding protein." in: PLoS ONE, Vol. 6, Issue 10, pp. e25884, (2012) (PubMed).

Spruijt, Bartels, Brinkman, Tjeertes, Poser, Stunnenberg, Vermeulen: "CDK2AP1/DOC-1 is a bona fide subunit of the Mi-2/NuRD complex." in: Molecular bioSystems, Vol. 6, Issue 9, pp. 1700-6, (2010) (PubMed).

Target Details for MBD2

Target: MBD2 (Methyl-CpG Binding Domain Protein 2 (MBD2))

Alternative Name: MBD2

Background: MBD2, methyl-CpG binding domain protein 2 , DKFZp586O0821, DMTase, NY-CO-41

Gene ID: 8932, 17191

NCBI Accession: NP_003918

Pathways: Chromatin Binding