PRPF31 antibody (Internal Region)

Catalog No. ABIN334465

This Goat Polyclonal antibody specifically detects PRPF31 in WB, ELISA, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for PRPF31 antibody (Internal Region) (ABIN334465)

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PRPF31 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-PRPF31 Antibody

Binding Specificity: Internal Region

Purpose: PRPF31

Sequence: KELGNSLDKC KNNEN

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KELGNSLDKCKNNEN, from the internal region of the protein sequence according to NP_056444.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in nuclear lysates of cell lines A431, HeLa, HEK293 and NIH3T3 (calculated MW of 55.4 kDa according to Human NP_056444.3 and Mouse NP_081604.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at roo

Peptide ELISA: antibody detection limit dilution 1:128000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HEK293 and HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PRPF31

Target: PRPF31 (Pre-mRNA Processing Factor 31 (PRPF31))

Alternative Name: PRPF31

Background: PRPF31, PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae), DKFZp566J153, NY-BR-99, PRP31, RP11 , PRP31 pre-mRNA processing factor 31 homolog (yeast), pre-mRNA processing factor 31 homolog, pre-mRNA processing factor 31 homolog (yeast)

Gene ID: 26121, 68988

NCBI Accession: NP_056444

Pathways: Ribonucleoprotein Complex Subunit Organization