SMEK1 antibody (Internal Region)

Catalog No. ABIN334493

The Goat Polyclonal anti-SMEK1 antibody has been validated for WB and ELISA. It is suitable to detect SMEK1 in samples from Human.

Quick Overview for SMEK1 antibody (Internal Region) (ABIN334493)

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SMEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-SMEK1 Antibody

Binding Specificity: Internal Region

Purpose: SMEK1 / KIAA2010

Sequence: EQQRERQDNP KLD

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EQQRERQDNPKLD, from the internal region of the protein sequence according to NP_115949.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 110 kDa band observed in Human Pancreas lysates (calculated MW of 93.9 kDa according to NP_115949.3). In transfected HEK293 transiently expressing SMEK1 a band of approx. 95 kDa is observed. This band is not observed in the non-transfec

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SMEK1

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Alternative Name: SMEK1

Background: SMEK homolog 1, suppressor of mek1 (Dictyostelium) , KIAA2010 , FLFL1 , MSTP033 , smk-1 , smk1, SMEK homolog 1, suppressor of mek1

Gene ID: 55671, 68734, 314388

NCBI Accession: NP_115949

Pathways: Regulation of Carbohydrate Metabolic Process