HNF1B antibody (Internal Region)

Catalog No. ABIN334505

The Goat Polyclonal anti-HNF1B antibody has been validated for WB, ELISA and ICC. It is suitable to detect HNF1B in samples from Human and Mouse. There is 1 publication available.

Quick Overview for HNF1B antibody (Internal Region) (ABIN334505)

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This HNF1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Grade: Verified

Product Details anti-HNF1B Antibody

Binding Specificity: Internal Region

Purpose: TCF2 / VHNF1

Sequence: QAYDRQKNPS KEER

Specificity: This antibody is expected to recognize both reported isoforms (NP_000449.1, NP_001159395.1). This product may cross-react with HNF1A.

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat, Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QAYDRQKNPSKEER, from the internal region of the protein sequence according to NP_000449.1, NP_001159395.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 60 kDa band observed in Human Liver lysates (calculated MW of 61.3 kDa according to NP_000449.1). Recommended concentration: 0.1-0.3 μg/mL. Primary incubation was 1 hour. An additional band of unknown identity was also consistently ob

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunocytochemistry: A customer found positive results in ICC on Human primitive gut tube from Human embryonic stem cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-HNF1B antibody (ABIN334505)

Arenas, Hervias, Uriz, Joplin, Prieto, Medina: "Combination of ursodeoxycholic acid and glucocorticoids upregulates the AE2 alternate promoter in human liver cells." in: The Journal of clinical investigation, Vol. 118, Issue 2, pp. 695-709, (2008) (PubMed).

Target Details for HNF1B

Target: HNF1B (HNF1 Homeobox B (HNF1B))

Alternative Name: HNF1B

Background: TCF2, VHNF1, transcription factor 2, hepatic LF-B3 variant hepatic nuclear factor, FJHN, HNF1B, HNF1beta, HNF2, LFB3, MODY5, transcription factor 2

Gene ID: 6928, 21410, 25640

NCBI Accession: NP_000449, NP_001159395

Pathways: Hormone Transport, Stem Cell Maintenance, Tube Formation