CP43' antibody

Catalog No. ABIN7540294

Cited in 1 publication. The Rabbit Polyclonal anti-CP43' antibody (ABIN7540294) specifically detects CP43' in WB. The antibody is reactive with Synechocystis samples.

Quick Overview for CP43' antibody (ABIN7540294)

Target: CP43'

Reactivity: Synechocystis

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Purpose: Anti-CP43' | IsiA homolog of plant CP43'

Cross-Reactivity (Details): Synechocystis sp. PCC6803
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Acaryochloris marina,Cyanophyta sp.,Chlamydomonas reinhardtii,Halomicronema hongdechloris,Nostoc sp.,Synechococcus elongatus PCC 7942,Synechocystis sp. PCC 6803,Thermosynechococcus elongatus

Purification: Antigen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide nearly perfectly conserved across known IsiA/CP43 proteins including Synechocystis PCC sp. 6803 CP43' Q55274

Application Details

Application Notes: 1 : 1000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Antigen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Schrader, Milligan, Behrenfeld: "Surplus photosynthetic antennae complexes underlie diagnostics of iron limitation in a cyanobacterium." in: PLoS ONE, Vol. 6, Issue 4, pp. e18753, (2011) (PubMed).

Target Details

Target: CP43'

Background:

Background: CP43' is encoded by the IsiA gene. This chlorophyll (Chl)-binding protein is induced under iron deficiency conditions in certain cyanobacterial strains. It acts as dissipater of light energy protecting photosystem II from excessive excitation under iron-deficient conditions.

Additional information: Peptide used to elicit this antibody is also perfectly or highly conserved in known Pcb chlorophyll a/b binding proteins from Prochlorococcus and similar proteins from other cyanobacteria. Peptide target is partially conserved in CP43/PsbC. CP43' and CP43 can be distinguished by their size.

Molecular Weight: 37 | 27 kDa (in a Novex gel system)

UniProt: Q55274