PsbF (Subunit beta) antibody

Catalog No. ABIN334540

This Rabbit Polyclonal antibody specifically detects PsbF in WB. It exhibits reactivity toward Arabidopsis thaliana, Pea and Triticum aestivum.It has been mentioned in 1 publication

Quick Overview for PsbF (Subunit beta) antibody (ABIN334540)

Target: PsbF

Reactivity: Arabidopsis thaliana, Pea, Triticum aestivum

Host: Rabbit

Clonality: Polyclonal

Application: Western Blotting (WB)

Product Details

Binding Specificity: Subunit beta

Purpose: Anti-PsbF | beta subunit of Cytochrome b559 of PSII

Cross-Reactivity (Details): Arabidopsis thaliana, Pisum sativum, Triticum aestivum
Not reactive in: Chlamydomonas reinhardtii, cyanobacteria

Predicted Reactivity: Cannabis sativa,Marchantia polymorpha,Nicotiana tabacum,Pinus sylvestris,Populus tremula

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide derived from plant PsbF sequences including Arabidopsis thaliana P62095

Application Details

Application Notes: 1 : 1000 (WB)

Comment:

This antibody works better on thylakoid and chloroplast fractions than on a total cell extract.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Luciński, Misztal, Samardakiewicz, Jackowski: "Involvement of Deg5 protease in wounding-related disposal of PsbF apoprotein." in: Plant physiology and biochemistry : PPB / Société française de physiologie végétale, Vol. 49, Issue 3, pp. 311-20, (2011) (PubMed).

Target Details

Target: PsbF

Background:

Background: Cytochrome b559 (Cyt b559) is encoded by the chloroplast genes psbE and psbF and is comprised of two low molecular mass polypeptides, α and ß subunits, with molecular masses of 9 and 4 kDa, respectively. The Cyt b559 is closely associated with PSII in all oxygenic photosynthetic organisms. The α and ß subunits of the Cyt b559 are components of the minimal PSII reaction center complex that is still capable of primary charge separation In summary, both PsbE and PsbF are essential components for PSII assembly, and they are probably involved in electron transport mechanisms that help to protect PSII from photodamage.

Additional information: This product can be sold containing ProClin if requested

Molecular Weight: 4 kDa

Gene ID: 844746

UniProt: P62095