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CDJ1 antibody (N-Term)

CDJ1 Reactivity: Chlamydomonas reinhardtii IP, WB Host: Rabbit Polyclonal unconjugated
Pubmed (2 references)
Catalog No. ABIN334548
$634.95
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100 μL
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  • Target
    CDJ1 (Chloroplast DnaJ Protein (CDJ1))
    Binding Specificity
    N-Term
    Reactivity
    Chlamydomonas reinhardtii
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Immunoprecipitation (IP), Western Blotting (WB)
    Cross-Reactivity (Details)
    Not reactive in: higher plants
    Predicted Reactivity
    Ostreococcus sp.
    Characteristics
    Expected / apparent Molecular Weight of the Antigene: 40 / 40 kDa
    Purification
    serum
    Immunogen
    fusion protein encoding region for the Chlamydomonas rerinhardtii N-terminal part of CDJ1 Q66YD3, overexpressed in E.coli
  • Application Notes
    1. 3000 with standard ECL (WB)
    Comment

    western blot has been done on 10-50 µg load per well, immunoprecipitation has been done on 5-50 µl

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    For reconstitution add 100 μL of sterile water.
    Handling Advice
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Long, Sommer, Allen, Lu, Merchant: "FER1 and FER2 encoding two ferritin complexes in Chlamydomonas reinhardtii chloroplasts are regulated by iron." in: Genetics, Vol. 179, Issue 1, pp. 137-47, (2008) (PubMed).

    Willmund, Dorn, Schulz-Raffelt, Schroda: "The chloroplast DnaJ homolog CDJ1 of Chlamydomonas reinhardtii is part of a multichaperone complex containing HSP70B, CGE1, and HSP90C." in: Plant physiology, Vol. 148, Issue 4, pp. 2070-82, (2008) (PubMed).

  • Target
    CDJ1 (Chloroplast DnaJ Protein (CDJ1))
    Alternative Name
    CDJ1
    Synonyms
    chloroplast DnaJ-like protein antibody, CDJ1 antibody
    Background
    CDJ1 is one of three zinc-finger containing J domain proteins of Chlamydomonas reinhardtii. This protein is found in chloroplast. In spite that CDJ1 gene is heat shock inducible it does not reflect in increased CDJ1 protein levels. CDJ1 forms complexes with plastidic HSP90C,HSP70B and CGE1 and is a new cohort protein of the chloroplast multi-chaperone complex.DnaJ/Hsp40 (heat shock protein 40) proteins are highly conserved and play crucial roles in protein translation, folding, unfolding, translocation, and degradation.
    Molecular Weight
    expected: 40 kDa, apparent: 40 kDa
    UniProt
    Q66YD3
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