50S Ribosomal Protein L30 antibody
Quick Overview for 50S Ribosomal Protein L30 antibody (ABIN7676204)
Target
Reactivity
Host
Clonality
Application
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Purpose
- Anti-L7/L12 | Ribosomal protein
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Cross-Reactivity (Details)
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Chlamydomonas reinhardtii, weakly reacts with the r-protein in spinach ca. 1 %
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Purification
- Serum
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Immunogen
- Purified native Chlamydomonas reinhardtii L-30 protein eluted from a gel piece
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Application Notes
- 1: 10 (IF), 1 : 1000 (WB)
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Comment
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Cross react with L2 and L26 proteins of Chlamydomonas reinhardtii. L7/L12 is very acidic, may not bind well to nitrocellulose membrane and can have abberant mobility depending upon conditions.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Serum
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- 50S Ribosomal Protein L30
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Alternative Name
- 50S ribosomal protein L30 (L-30)
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Background
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Background: Ribosomal protein L-30 is a part of 50S ribosomal large subunit, synthesized in chloroplast. Schmidt et al. (1983) Sites of synthesis of chloroplast ribosomal proteins in Chlamydomonas. J Cell Biol 96(5):1451-1463
Additional information: Name of this antibody has been changed from L-30 | 50S ribosomal protein L30 to L7/L12 based on the following reference: Randolph-Anderson et al. (1989). Electrophoretic and immunological comparisons of chloroplast and prokaryotic ribosomal proteins reveal that certain families of large subunit proteins are evolutionarily conserved. J Mol Evol. 1989 Jul,29(1):68-88.
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Molecular Weight
- 11.9 kDa (Chlamydomonas reinhardtii), 15.7 kDa (spinach)
Target
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