HSP70 antibody (Cytoplasmic)

Catalog No. ABIN334584

Cited in 4+ publications. The Rabbit Polyclonal anti-HSP70 antibody (ABIN334584) specifically detects HSP70 in WB and IP. The antibody is reactive with Plasmodium falciparum, Barley, Arabidopsis thaliana, Pinus strobus, Rape, Cabbage, Chlamydomonas reinhardtii, Alfalfa, Algae (Desmodesmus subspicatus), Chlamydomonas, Fagopyrum esculentum, Cucumis sativus, Silene vulgaris, Gracilaria vermiculophylla, Mustard, Picrorhiza kurroa, Eragrostis tef, Polyscias elegans, Salicornia, Setaria italica, Zea mays, Soybean, Tomato, Triticum aestivum, Ulva prolifera and Vicia faba samples.

Quick Overview for HSP70 antibody (Cytoplasmic) (ABIN334584)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Plasmodium falciparum, Barley, Arabidopsis thaliana, Pinus strobus, Rape, Cabbage, Chlamydomonas reinhardtii, Alfalfa, Algae (Desmodesmus subspicatus), Chlamydomonas, Fagopyrum esculentum, Cucumis sativus, Silene vulgaris, Gracilaria vermiculophylla, Mustard, Picrorhiza kurroa, Eragrostis tef, Polyscias elegans, Salicornia, Setaria italica, Zea mays, Soybean, Tomato, Triticum aestivum, Ulva prolifera, Vicia faba

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-HSP70 Antibody

Binding Specificity: Cytoplasmic

Purpose: Anti-HSP70 | Heat shock protein 70 (cytoplasmic)

Cross-Reactivity (Details): Arabidopsis thaliana, Brassica juncea, Brassica napus, Brassica oleracea, Chlamydomonas reinhardtii, Chlamydomonas sp. UWO241, Cucumis sativus, E. teft, Fagopyrum esculentum, Glycine max, Hordeum vulgare, Medicago sativa, Salicornia sp., Silene vulgaris, Solanum lycopersicum, Picrorhiza kurroa, Pinus strobus, Polyscias elegans, Zea mays, algae: Desmodesmus subspicatus, Gracilaria vermiculophylla, phycobiont: Trebouxia TR1 and TR9, Plasmodium falciparum, Setaria italica, Triticum aestivum, Ulva prolifera, Vicia faba
Not reactive in: Centella asiatica, Polyscias elegans

Predicted Reactivity: Ageratina adenophora,Allium sativum,Arabis alpina,Arachis diogoi,Arundo donax,Brassica napus,brassica rapa subsp. pekinensis,Camellia sinensis,Chlorella vulgaris,Citrus clementina,Citrus sinensis,Coffea arabica,Eriobotrya japonica,Gossypium arboretum,Glycine soja,Helianthus annuus,Hordeum vulgare var. distichum,Lotus japonicus,Medicago sativa,medicago truncatula,Musa acuminata subsp. malaccensis,Nannochloropsis gaditana,Nicotiana tabacum,Nicotiana bethamiana,Olea europea,Oryza sativa,Phaseolus vulgaris,Physcomitrium patens,Pinus taeda,Pisum sativum,Populus balsamifera,Populus trichocarpa,Saccharum sp,Salix gilgiana,Saussurea medusa,Solanum tuberosum,Solanum commersonii,Spinacia oleracea,Tragopogon dubius,Tragopogon porrifolius,Triticum aestivum,Vitis vinifera,Volvox sp.

Purification: Serum

Immunogen: KLH-conjugated synthetic peptide conserved in known higher plant HSC70 proteins including three isoforms of Arabidopsis thaliana HSC70-1 UniProt: F4KCE5

Application Details

Application Notes: 1 : 3000 - 1: 10 000, 5 μg protein/well (WB), 2-3 μL/protein extract of concentration 3-5 mg/mL

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References for anti-HSP70 antibody (ABIN334584)

Norhayati, Hazlina, Asrenee, Emilin: "Magnitude and risk factors for postpartum symptoms: a literature review." in: Journal of affective disorders, Vol. 175, pp. 34-52, (2015) (PubMed).

Muralidharan, Oksman, Pal, Lindquist, Goldberg: "Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers." in: Nature communications, Vol. 3, pp. 1310, (2012) (PubMed).

Alvarez, del Hoyo, García-Breijo, Reig-Armiñana, del Campo, Guéra, Barreno, Casano: "Different strategies to achieve Pb-tolerance by the two Trebouxia algae coexisting in the lichen Ramalina farinacea." in: Journal of plant physiology, Vol. 169, Issue 18, pp. 1797-806, (2012) (PubMed).

Janicka-Russak, Kaba?a, Burzynski: "Different effect of cadmium and copper on H+-ATPase activity in plasma membrane vesicles from Cucumis sativus roots." in: Journal of experimental botany, Vol. 63, Issue 11, pp. 4133-42, (2012) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background:

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and is highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery. Heat shock cognate protein 70 (HSC70), is a highly conserved protein and a member of the family of molecular chaperones.

Additional information: This product can be sold containing ProClin if requested

Molecular Weight: 70 kDa

Gene ID: 820438

NCBI Accession: NP_187555, NP_195869

UniProt: Q9LHA8