Ascorbate Oxidase antibody (Biotin)

Catalog No. ABIN334591

This Rabbit Polyclonal antibody specifically detects Ascorbate Oxidase in ELISA and WB. It exhibits reactivity toward Cucurbita pepo.

Quick Overview for Ascorbate Oxidase antibody (Biotin) (ABIN334591)

Target: Ascorbate Oxidase (AO)

Reactivity: Cucurbita pepo

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ascorbate Oxidase antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB)

Product Details anti-Ascorbate Oxidase Antibody (Biotin)

Purpose: Anti-AO | ascorbate oxidase, Biotin conjugated

Cross-Reactivity (Details): Cucurbita sp.
Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Predicted Reactivity: Glycne max,Oryza sativa

Purification: Purified IgG

Immunogen: ascorbate oxidase purified from Cucurbita sp.

Isotype: IgG

Application Details

Application Notes: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)

Comment:

IgG concentration is 10 mg/ml. Biotin/IgG protein molar ratio is approximately 5.6. No foreign proteins are added.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 1 mL of sterile water, Allow reconstituted product to stand for at least 30 minutes at room temperature prior to dilution, If necessary, centrifuge to remove any particulates, Prepare fresh working dilution daily

Buffer: Purified IgG in PBS pH 7.2.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.

Target Details for Ascorbate Oxidase

Target: Ascorbate Oxidase (AO)

Background:

Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA). Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90 % of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.

Additional information: IgG protein fraction is prepared by ammonium sulphate precipitation and ion exchanged chromatography, N-Hydroxysuccinimidobiotin is used for labelling of antibody

Molecular Weight: 61 | 45 kDa (Cucurbita pepo)