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Ascorbate Oxidase antibody (Biotin)

AO Reactivity: Pumpkin ELISA, WB, IP Host: Rabbit Polyclonal Biotin
Catalog No. ABIN1607781
  • Target See all Ascorbate Oxidase (AO) products
    Ascorbate Oxidase (AO)
    Reactivity
    • 7
    • 2
    Pumpkin
    Host
    • 9
    Rabbit
    Clonality
    • 9
    Polyclonal
    Conjugate
    • 5
    • 3
    • 1
    This Ascorbate Oxidase antibody is conjugated to Biotin
    Application
    • 8
    • 8
    • 3
    • 2
    • 2
    • 2
    ELISA, Western Blotting (WB), Immunoprecipitation (IP)
    Cross-Reactivity (Details)
    Cross reactivity against Ascorbate Oxidase from other sources may occur but have not been specifically determined.
    Purity
    Anti-Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ascorbate Oxidase [cucurbita].
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Ascorbate Oxidase [cucurbita]
    Immunogen Type: Native Protein
    Isotype
    IgG
  • Application Notes
    Anti-Ascorbate Oxidase has been assayed against 1.0 µg of Ascorbate Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2.000 to 1:10.000 of the reconstitution concentration is suggested for this product.
    ELISA Dilution: 1:5.000 - 1:20.000
    IF Immunoprecipitation Dilution: 1:100
    Western Blot Dilution: 1:500 - 1:5.000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    10 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    Ascorbate Oxidase (AO)
    Alternative Name
    Ascorbate Oxidase (AO Products)
    Synonyms
    AO antibody, ascorbate oxidase antibody, L-ascorbate oxidase antibody, AO antibody, LOC9310099 antibody
    Background
    Anti-Ascorbate Oxidase antibody is cytoplasmic and performs binding with copper ions. Anti-Ascorbate Oxidase belongs to the multicopper oxidase family and may be a factor in the redox system involving ascorbic acid, acting as a catalyst. Found prevalently in plants, Ascorbate Oxidase has been suggested to increase plant growth. Anti-Ascorbate Oxidase antibody is ideal for researchers interested in the investigation of Cell Biology. Synonyms: L-ascorbate oxidase Ascorbase ASO EC=1.10.3.3
    UniProt
    P24792
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