EWSR1 antibody (Internal Region)

Catalog No. ABIN335135

Product Details anti-EWSR1 Antibody

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EWSR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: EWS / EWSR1

Sequence: TSYDQSSYSQ QNTYG

Specificity: This antibody is expected to recognize all five reported isoforms (NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1).

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-TSYDQSSYSQQNTYG, from the internal region of the protein sequence according to NP_053733.2, NP_005234.1, NP_001156757.1, NP_001156758.1, NP_001156759.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5-7 μg/mL.

Western Blot: Approx. 90 kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 68.5 kDa according to NP_053733.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for EWSR1

Target: EWSR1 (Ewing Sarcoma Breakpoint Region 1 (EWSR1))

Alternative Name: EWSR1 (EWSR1 Products)

Background: EWSR1, EWS, Ewing sarcoma breakpoint region 1, EWS/FLI fusion protein, EWSR1/ZNF384 fusion protein, Ewings sarcoma EWS-Fli1 (type 1) oncogene, bK984G1.4 (Ewing sarcoma breakpoint region 1 protein), extraskeletal myxoid chondrosarcoma EWS/TEC/CHN fusion pr

Gene ID: 2130, 14030, 289752

NCBI Accession: NP_053733, NP_005234, NP_001156757, NP_001156758, NP_001156759