Notch1 antibody (Internal Region)

Catalog No. ABIN335152

This Goat Polyclonal antibody specifically detects Notch1 in ELISA. It exhibits reactivity toward Zebrafish (Danio rerio).

Quick Overview for Notch1 antibody (Internal Region) (ABIN335152)

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Reactivity: Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This Notch1 antibody is un-conjugated

Application: ELISA

Grade: Recent

Product Details anti-Notch1 Antibody

Binding Specificity: Internal Region

Purpose: Notch1a (zebrafish)

Sequence: QNMAPMQHQN ISHH

Cross-Reactivity: Zebrafish (Danio rerio)

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: C-QNMAPMQHQNISHH

Isotype: IgG

Application Details

Application Notes:

Western Blot: Not yet tested - our routine western blotting protocol does not allow for the detection of proteins >250 kDa (calculated size of approx. 262 kDa according to NP_571516.1 ) and the antibody is an aspiring product.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for Notch1

Target: Notch1 (NOTCH1) (Notch 1 (NOTCH1))

Alternative Name: notch1a

Background: Notch1a, notch homolog 1a , n1a, deadly seven, des, neurogenic locus notch homolog protein

NCBI Accession: NP_571516

Pathways: Notch Signaling, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development