NR1I2 antibody (Internal Region)

Catalog No. ABIN335159

The Goat Polyclonal anti-NR1I2 antibody has been validated for WB, IHC and ELISA. It is suitable to detect NR1I2 in samples from Human.

Quick Overview for NR1I2 antibody (Internal Region) (ABIN335159)

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NR1I2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-NR1I2 Antibody

Binding Specificity: Internal Region

Purpose: PXR / NR1I2

Sequence: EQFAITLKSY IECNR

Specificity: This antibody is expected to recognize all reported isoforms (NP_071285.1, NP_148934.1, NP_003880.3).

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence EQFAITLKSYIECNR, from the internal region of the protein sequence according to NP_003880.3, NP_071285.1, NP_148934.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Small Intestine. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx. 48 kDa band observed in lysates of cell line HepG2 (calculated MW of 49.8 kDa according to NP_003880.3). Recommended concentration: 1-3 μg/mL. An additional band of unknown identity was also consistently observed at 35 kDa. This band wa

Peptide ELISA: antibody detection limit dilution 1:4000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for NR1I2

Target: NR1I2 (Nuclear Receptor Subfamily 1, Group I, Member 2 (NR1I2))

Alternative Name: NR1I2

Background: PXR, NR1I2, nuclear receptor subfamily 1, group I, member 2, BXR, ONR1, PAR, PAR1, PAR2, PARq, PRR, SAR, SXR, orphan nuclear receptor, pregnane X receptor, steroid and xenobiotic receptor

Gene ID: 8856

NCBI Accession: NP_003880, NP_071285, NP_148934

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway