SYT1 antibody (Internal Region)

Catalog No. ABIN335168

The Goat Polyclonal anti-SYT1 antibody has been validated for WB, IHC and ELISA. It is suitable to detect SYT1 in samples from Human.

Quick Overview for SYT1 antibody (Internal Region) (ABIN335168)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SYT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Grade: Verified

Product Details anti-SYT1 Antibody

Binding Specificity: Internal Region

Purpose: Synaptotagmin I

Sequence: RDLQSAEKEE QEK

Specificity: Reported isoforms NP_005630.1, NP_001129277.1 and NP_001129278.1 represent identical protein.

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDLQSAEKEEQEK, from the internal region of the protein sequence according to NP_005630.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon. Recommended concentration: 4-6 μg/mL.

Western Blot: Approx. 65 kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6 kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibod

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: SYT1

Background: Synaptotagmin I , DKFZp781D2042, SYT1, P65, SVP65, SYT, synaptotagmin 1

Molecular Weight: 47.6kDa

Gene ID: 6857, 20979, 25716

NCBI Accession: NP_005630

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport