Desmin antibody

Catalog No. ABIN335365

Product Details anti-Desmin Antibody

Target: Desmin (DES)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Desmin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Human.

Purification: Purified

Immunogen: D9 is a mouse monoclonal IgG1 antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with desmin isolated from human leiomyoma.

Clone: D9

Isotype: IgG1

Application Details

Application Notes: D9 reacts exclusively with desmin, which is expressed in smooth and striated muscle cells and their tumors e.g. rhabdomyosarcoma and leiomyosarcoma. D9 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. Optimal antibody dilution should be determined by titration, recommended range is 1:25 - 1:200 for immunohistochemistry with avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

Restrictions: For Research Use only

Handling

Storage: 4 °C

References for anti-Desmin antibody (ABIN335365)

Council, Hameed: "Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma." in: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, Vol. 22, Issue 5, pp. 639-50, (2009) (PubMed).

Target Details for Desmin

Target: Desmin (DES)

Alternative Name: Desmin (DES Products)

Synonyms: DES antibody, des-b antibody, MGC80853 antibody, des antibody, csm1 antibody, csm2 antibody, desm antibody, cmd1i antibody, MGC75911 antibody, LOC100220724 antibody, desmin antibody, cb290 antibody, fb59a12 antibody, wu:fb59a12 antibody, zgc:109859 antibody, CSM1 antibody, CSM2 antibody, LGMD2R antibody, wu:fc11d08 antibody, zgc:154009 antibody, des-a antibody, MGC52614 antibody, desmin antibody, desmin, gene 1 L homeolog antibody, desmin, gene 1 antibody, desmin a antibody, desmin b antibody, desmin, gene 1 S homeolog antibody, desmin, gene 2 S homeolog antibody, DES antibody, des.1.L antibody, des.1 antibody, des antibody, desma antibody, Des antibody, desmb antibody, des.1.S antibody, des.2.S antibody

Background: Desmin (53 kDa) exhibits a high degree of tissue specificity, its expression being predominantly confined to all types of muscle cells (cardiac, skeletal and smooth muscle). Regulation of desmin expression is stage and tissue-specific, since it is induced during terminal development of, for example, skeletal muscle cell differentiation. In skeletal en cardiac muscle cells desmin is localized in the Z-disk region and at the intercalated disk. The expression pattern of desmin in smooth muscle is much more heterogenous. Coexpression of vimentin and desmin has been observed in tumors derived from muscle tissue, i.e. rhabdomyosarcomas and leiomyosarcomas. Furthermore, during myocard dysfunction dramatic changes in the distribution of desmin have been observed.