NFATC1 antibody (AA 428-716)

Catalog No. ABIN336112

The Mouse Monoclonal anti-NFATC1 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect NFATC1 in samples from Human.

Quick Overview for NFATC1 antibody (AA 428-716) (ABIN336112)

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NFATC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: AT1C3

Product Details anti-NFATC1 Antibody

Binding Specificity: AA 428-716

Specificity: The antibody recognizes Human NFATc1. Other species not tested.

Characteristics: Synonyms: NF-ATc1, NFATC1, NFATC, Nuclear factor of activated T-cells, cytoplasmic 1

Purification: Protein-G affinity chromatography

Immunogen: Recombinant human NFATc1 (aa 428-716) purified from E. coli

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western blot (1/1,000-1/2,000). Immunohistochemistry on Paraffin Sections (10 μg/mL). This NFATC1 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % Sdium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for up to two weeks or (in aliquots) at -20 °C or -70 °Cfor longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for NFATC1

Target: NFATC1 (Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1))

Alternative Name: NFAT2 / NFATC

Background: Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1 (NFATc1) is a component of the nuclear factor of activated T cells DNA-binding transcription complex which consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation, and an inducible nuclear component. This protein plays a main role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 or IL-4 gene transcription and functions as a major molecular target for the immunosuppressive drugs such as cyclosporine A. NFATc1 is expressed in most human primary lymphocytes and mature human T- and B-cell neoplasms.Synonyms: NF-ATc1, NFATC, NFATC1, Nuclear factor of activated T-cells, cytoplasmic 1

Gene ID: 4772

UniProt: O95644

Pathways: RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway