Cytokeratin (LMW) antibody

Catalog No. ABIN336874

This Mouse Monoclonal antibody specifically detects Cytokeratin (LMW) in IHC and IHC (p). It exhibits reactivity toward Human, Rat, Chicken, Cow, Monkey, Mouse, Rabbit and Pig.

Quick Overview for Cytokeratin (LMW) antibody (ABIN336874)

Target: Cytokeratin (LMW)

Reactivity: Human, Rat, Chicken, Cow, Monkey, Mouse, Rabbit, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: AE1

Product Details

Specificity: Molecular Weight: (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19 (40).

Immunogen: Human epithelial keratin

Isotype: IgG1

Application Details

Application Notes: IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. For IHC dilute conc. antibodies 1:50-1:100, use streptavidin-biotin system or polymer system, incubate 30 minutes at room temperature. Prediluted antibody is ready to be used for IHC. This antibody requires antigen retriever, Trypsin (AR-6541) or citrate buffer (AR-6544). (Follow data sheet for Trypsin, or boil tissue in 10 mM citrate, pH 6.0 for 10-15 minutes followed by cooling for 10-15 minutes at RT). The optimum dilution should be determined by the individual lab. Positive Control: Human skin, squamous cell Ca. Cellular Localization: Cytoplasmic.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The concentrated antibodies are supplied in PBS with 1% BSA, 0.05% azide, pH 7.4. The Prediluted antibody is supplied in our Universal antibody dilution buffer (AR-6526) green in color.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details

Target: Cytokeratin (LMW)

Alternative Name: Cytokeratin Low MW Keratins

Background: Cytokeratins are intermediate filament keratins found in the intracytoplasmic cytoskeleton of epithelial tissue There are two types of Cytokeratins: the low weight, acidic type I cytokeratins and the high weight, basic or neutral type II. Cytokeratins are usually found in pairs comprising a type I Cytokeratin and a type II cytokeratin. The high molecular weight cytokeratins, which are the basic or neutral cytokeratins, comprise subtypes CK1, CK2, CK3, CK4, CK5, CK6, CK7, CK8 and CK9. The low molecular weight cytokeratins, which are the acidic cytokeratins, comprise subtypes CK10, CK12, CK 13, CK14, CK16, CK17, CK18, CK19 and CK20.This antibody recognizes low molecular weight cytokeratins (CK 10 (56.5), CK14 (50), CK15 (50), CK16 (48) and CK19(40) of the acidic family.