Dynactin 1 antibody (AA 1266-1278)

Catalog No. ABIN337005

The Goat Polyclonal anti-Dynactin 1 antibody (ABIN337005) specifically detects Dynactin 1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Horse, Monkey and Hamster samples.

Quick Overview for Dynactin 1 antibody (AA 1266-1278) (ABIN337005)

Target: Dynactin 1 (DCTN1)

Reactivity: Human, Mouse, Horse, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Dynactin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Dynactin 1 Antibody

Binding Specificity: AA 1266-1278

Specificity: Human DCTN1. This antibody is expected to recognise all reported isoforms (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%) Rat, Bovine, Dog, Panda, Rabbit, Pig (92%) Bat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-QEQLHQLHSRLIS from the C-terminus of human DCTN1 (NP_004073.2, NP_075408.1, NP_001128512.1, NP_001128513.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Horse (100%), Rat, Bovine, Dog, Panda, Rabbit, Pig (92%), Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:128000), WB (1:128000, 0.5 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Dynactin 1

Target: Dynactin 1 (DCTN1)

Alternative Name: DCTN1 / Dynactin 1

Background: Name/Gene ID: DCTN1

Synonyms: DCTN1, DAP-150, Dynactin subunit 1, DP-150, Dynactin 1, HMN7B, p150-glued, p135

Gene ID: 1639

NCBI Accession: NP_004073, NP_075408, NP_001128512, NP_001128513

UniProt: Q14203

Pathways: M Phase, ER-Nucleus Signaling