Midline 2 antibody (AA 723-735)

Catalog No. ABIN337007

The Goat Polyclonal anti-Midline 2 antibody is suitable to detect Midline 2 in samples from Human, Mouse and Monkey. It has been validated for WB, ELISA, IHC and IHC (p).

Quick Overview for Midline 2 antibody (AA 723-735) (ABIN337007)

Target: Midline 2 (MID2)

Reactivity: Human, Mouse, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This Midline 2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Midline 2 Antibody

Binding Specificity: AA 723-735

Specificity: Human MID2. This antibody is expected to recognise both human isoforms of this protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%) Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%) Hamster, Rabbit, Zebrafish, Stickleback (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide QESPYVSGMKTCH from the C-terminus of human MID2 (NP_036348.2, NP_438112.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset (100%), Mouse, Rat, Dog, Bat, Elephant, Panda, Horse, Pig, Opossum, Turkey, Chicken (92%), Hamster, Rabbit, Zebrafish, Stickleback (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Midline 2

Target: Midline 2 (MID2)

Alternative Name: TRIM1 / MID2

Background: Name/Gene ID: MID2

Synonyms: MID2, FXY2, Midin-2, Midline-2, Midin 2, Midline 2, Midline defect 2, RNF60, RING finger protein 60, TRIM1, Tripartite motif protein 1

Gene ID: 11043

NCBI Accession: NP_036348, NP_438112

UniProt: Q9UJV3