F2RL1 antibody (N-Term)

Catalog No. ABIN337049

The Mouse Monoclonal anti-F2RL1 antibody has been validated for IHC, ELISA, IF and IHC (p). It is suitable to detect F2RL1 in samples from Human.

Quick Overview for F2RL1 antibody (N-Term) (ABIN337049)

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This F2RL1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SAM11

Product Details anti-F2RL1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes native peptide and the N-terminus of the human PAR2 receptor. Does not cross-react with PAR1.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to the N-terminal of the human PAR2 receptor.

Type of Immunogen: Synthetic peptide

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Immunofluorescence: 1-3 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for F2RL1

Target: F2RL1 (Coagulation Factor II (thrombin) Receptor-Like 1 (F2RL1))

Alternative Name: F2RL1 / PAR2

Background: Name/Gene ID: F2RL1
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2RL1, PAR2, Thrombin receptor-like 1, Protease-activated receptor 2, G-protein coupled receptor 11, GPR11, PAR-2

Gene ID: 2150

UniProt: P55085

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, SARS-CoV-2 Protein Interactome