TLR9 antibody (AA 268-284)

Catalog No. ABIN337069

This Mouse Monoclonal antibody specifically detects TLR9 in WB, IHC, FACS, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for TLR9 antibody (AA 268-284) (ABIN337069)

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR9 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 26C593-2

Product Details anti-TLR9 Antibody

Binding Specificity: AA 268-284

Brand: IHC-plus™

Specificity: KLH-conjugated synthetic peptide corresponding to amino acids 268-284 of human TLR9 isoform A (Genbank accession no. AAF78037).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Chimpanzee, Marmoset (94%) Sheep, Goat, Zebu, Bovine, Horse (82%).

Purification: Protein G purified

Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to amino acids 268-284 (CPRHFPQLHPDTFSHLS) of human TLR9 isoform A (Genbank accession no. AAF78037). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Chimpanzee, Marmoset (94%), Sheep, Goat, Zebu, Bovine, Horse (82%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr (10 - 20 μg/mL), IHC-P (5 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TLR9

Target: TLR9 (Toll-Like Receptor 9 (TLR9))

Alternative Name: TLR9

Background: Name/Gene ID: TLR9
Family: Toll-like Receptor

Synonyms: TLR9, CD289, CD289 antigen, Scri2a, Toll-like receptor 9

Gene ID: 54106

UniProt: Q9NR96

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades