TPSAB1 antibody

Catalog No. ABIN337075

This Mouse Monoclonal antibody specifically detects TPSAB1 in WB, IHC, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human, Rat, Dog, Cat and Monkey.

Quick Overview for TPSAB1 antibody (ABIN337075)

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Reactivity: Human, Rat, Dog, Cat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TPSAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: AA1

Product Details anti-TPSAB1 Antibody

Specificity: Recognizes human mast cell tryptase, both isoforms. Is an excellent marker for mast cells, and does not bind to any other cell type in immunohistology. Tryptases are the products of a number of and form the major neutral protease present in mast cells secreted in response to infection and injury. Mast cell tryptase has an important role in the pathology of inflammatory diseases, especially asthma through bronchoconstriction.

Purification: Protein G purified

Immunogen: Human mast cell tryptase purified from human lung tissue.

Type of Immunogen: Purified protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, IHC-P (2.5 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Histology Positive Control Tissue: Tonsil.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Target Details for TPSAB1

Target: TPSAB1 (Tryptase alpha/beta 1 (TPSAB1))

Alternative Name: TPSAB1 / Mast Cell Tryptase

Background: Name/Gene ID: TPSAB1
Subfamily: Serine S1
Family: Protease

Synonyms: TPSAB1, Alpha tryptase, Mast cell alpha II tryptase, Tryptase alpha-1, Tryptase beta I, Tryptase-III, TPSB1, Tryptase alpha II, TPS1, TPS2, Tryptase beta-1, Tryptase-1, Tryptase-I, Mast cell beta I tryptase, Tryptase alpha/beta 1, Tryptase alpha/beta-1, Tryptase beta 1, Tryptase I

Gene ID: 7177

UniProt: Q15661