CTNNB1 antibody (C-Term)
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- Target See all CTNNB1 Antibodies
- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat, Chicken, Xenopus laevis
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CTNNB1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Brand
- IHC-plus™
- Specificity
- Recognizes human b-catenin. Species cross-reactivity: chicken, mouse, rat and Xenopus.
- Purification
- Immunoaffinity purified
- Immunogen
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A 15 amino acid synthetic peptide derived from the C-terminus of the human and mouse b-catenin protein.
Type of Immunogen: Synthetic peptide - Isotype
- IgG
- Top Product
- Discover our top product CTNNB1 Primary Antibody
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- Application Notes
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Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (2.5 μg/mL), IP, WB
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL. Western Blot after IP: 1-2 μg/mL. Cross-reactivity with a ~100kD protein of unknown identity is sometimes observed. Immunoprecipitation: 5-10 μg. Specifically immunoprecipitate the ~92kD b-Catenin protein from native cell lysates. Positive control: HeLa and A431 lysates. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
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- Target
- CTNNB1 (Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1))
- Alternative Name
- CTNNB1 / Beta Catenin (CTNNB1 Products)
- Synonyms
- Jup antibody, LOC100217813 antibody, CTNNB1 antibody, CTNNB antibody, Bfc antibody, Catnb antibody, Mesc antibody, ctnnb antibody, id:ibd2058 antibody, wu:fb73e10 antibody, wu:fi81c06 antibody, wu:fk25h01 antibody, ctnnb1 antibody, CHBCAT antibody, MRD19 antibody, armadillo antibody, beta-catenin antibody, catenin beta 1 antibody, catenin (cadherin associated protein), beta 1 antibody, catenin (cadherin-associated protein), beta 1 antibody, catenin beta 1 S homeolog antibody, catenin beta 1 L homeolog antibody, CTNNB1 antibody, Ctnnb1 antibody, ctnnb1 antibody, ctnnb1.S antibody, ctnnb1.L antibody
- Background
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Name/Gene ID: CTNNB1
Family: Catenin
Synonyms: CTNNB1, Beta-catenin, Catenin beta-1, CTNNB, Beta Catenin - Gene ID
- 1499
- UniProt
- P35222
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Tube Formation, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2
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