Tropomyosin antibody

Catalog No. ABIN337101

The Mouse Monoclonal anti-Tropomyosin antibody (ABIN337101) specifically detects Tropomyosin in IHC, IF and IHC (p). The antibody is reactive with Human, Rat and Chicken samples.

Quick Overview for Tropomyosin antibody (ABIN337101)

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Reactivity: Human, Rat, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tropomyosin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Tropomyosin Antibody

Specificity: Recognizes chicken Tropomyosin. Reacts strongly with striated muscle forms of tropomyosin, including cardiac alpha- tropomyosin and skeletal alpha-and beta-tropomyosins. Species cross-reactivity: human and rat.

Purification: Protein G purified

Immunogen: Tropomyosin protein purified from chicken embryo fibroblasts (CEF) and chicken leg, heart, and gizzard.

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF, IHC, IHC-P (5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM PBS,  pH 7.4, 0.2 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Tropomyosin

Target: Tropomyosin (TPM1) (Tropomyosin 1 (Alpha) (TPM1))

Alternative Name: Tropomyosin

Pathways: Regulation of Actin Filament Polymerization