CLIC4 antibody (AA 8-20)

Catalog No. ABIN337111

This Goat Polyclonal antibody specifically detects CLIC4 in WB, IHC, ELISA, IF and IHC (p). It exhibits reactivity toward Human, Mouse, Rat and Monkey.

Quick Overview for CLIC4 antibody (AA 8-20) (ABIN337111)

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Reactivity: Human, Mouse, Rat, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This CLIC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CLIC4 Antibody

Binding Specificity: AA 8-20

Specificity: Human CLIC4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat (100%) Bovine (92%) Dog (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide NGLKEEDKEPLIE-C from the N-terminus of human CLIC4 (NP_039234.1). Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat (100%), Bovine (92%), Dog (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IF (5 μg/mL), IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.1 - 0.3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CLIC4

Target: CLIC4 (Chloride Intracellular Channel 4 (CLIC4))

Alternative Name: CLIC4

Background: Name/Gene ID: CLIC4
Subfamily: Chloride channel - intracellular
Family: Ion Channel

Synonyms: CLIC4, CLIC4L, DKFZP566G223, Mc3s5, HuH1, MTCLIC, p64H1

Gene ID: 25932

NCBI Accession: NP_039234

UniProt: Q9Y696