HPSE antibody

Catalog No. ABIN337167

The Rabbit Polyclonal anti-HPSE antibody (ABIN337167) specifically detects HPSE in WB, IHC and IHC (p). The antibody is reactive with Human and Mouse samples.

Quick Overview for HPSE antibody (ABIN337167)

Target: HPSE (Heparanase (HPSE))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPSE antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HPSE Antibody

Specificity: Recognizes the 65kD precursor as well as the 50kD and 8kD subunits of human or mouse Heparanase.

Purification: Protein G purified

Immunogen: A heterodimeric recombinant protein consisting of the 50 kDa and 8 kDa subunits.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 1:1000.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for HPSE

Target: HPSE (Heparanase (HPSE))

Alternative Name: HPSE / Heparanase

Background: Name/Gene ID: HPSE

Synonyms: HPSE, Endo-glucoronidase, Heparanase-1, Heparanase, HPA, HSE1, HEP, HPA1, HPSE1, HPR1

Gene ID: 10855

UniProt: Q9Y251

Pathways: Glycosaminoglycan Metabolic Process