APOA1 antibody

Catalog No. ABIN337201

This Goat Polyclonal antibody specifically detects APOA1 in WB, IHC, ELISA, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for APOA1 antibody (ABIN337201)

Target: APOA1 (Apolipoprotein A-I (APOA1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APOA1 Antibody

Specificity: Recognizes human Apolipoprotein A-I, minimum cross-reactivity against Type A-II, B, C-I, C-II, C-III, E and J Apolipoproteins and other human serum proteins.

Purification: Immunoaffinity purified

Immunogen: Highly purified Apolipoprotein Type A-I from human plasma.

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:10000), IHC, IHC-P (5 μg/mL), IP, WB (1:2500 - 1:5000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: BBS,  pH 8.0, 0.005 M EDTA, 0.01 % sodium azide, 40 % glycerol. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for APOA1

Target: APOA1 (Apolipoprotein A-I (APOA1))

Alternative Name: APOA1 / Apolipoprotein A 1

Background: Name/Gene ID: APOA1

Synonyms: APOA1, ApoA-I, APOAI, Apolipoprotein A-I, Apolipoprotein A1, Apo-AI

Gene ID: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism