SKP2 antibody

Catalog No. ABIN337213

This Mouse Monoclonal antibody specifically detects SKP2 in WB, ELISA, IHC, IP and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SKP2 antibody (ABIN337213)

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SKP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SKP2 Antibody

Brand: IHC-plus™

Specificity: Recognizes only human p45SKP2. Cross-reactivity with related endogenous proteins has not been observed.

Purification: Protein A purified

Immunogen: Recombinant, full-length human p45SKP2 protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC, IHC-P (5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Positive control: HeLa, Jurkat and 293T cell lines.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SKP2

Target: SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

Alternative Name: SKP2

Background: Name/Gene ID: SKP2

Synonyms: SKP2, F-box/LRR-repeat protein 1, FBL1, FLB1, F-box protein Skp2, FBXL1, p45, p45skp2

Gene ID: 6502

UniProt: Q13309

Pathways: Mitotic G1-G1/S Phases