SIRT4 antibody (AA 302-314)

Catalog No. ABIN337219

This Goat Polyclonal antibody specifically detects SIRT4 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for SIRT4 antibody (AA 302-314) (ABIN337219)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This SIRT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-SIRT4 Antibody

Binding Specificity: AA 302-314

Specificity: Human SIRT4.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Bovine, Horse, Pig (92%) Dog, Platypus (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide SRCGELLPLIDPC from the C-terminus of human SIRT4 (NP_036372.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon, Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Bovine, Horse, Pig (92%), Dog, Platypus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA, WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for SIRT4

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: SIRT4 / Sirtuin 4

Background: Name/Gene ID: SIRT4
Family: ADP-Ribosyltransferase

Synonyms: SIRT4, Sir2-like 4, SIR2-like protein 4, Sirtuin 4, Sirtuin type 4, SIR2L4

Gene ID: 23409

NCBI Accession: NP_036372

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion