CLDN14 antibody (AA 227-239)

Catalog No. ABIN337255

This Goat Polyclonal antibody specifically detects CLDN14 in WB, ELISA, IHC and IHC (p). It exhibits reactivity toward Human, Monkey and Rabbit.

Quick Overview for CLDN14 antibody (AA 227-239) (ABIN337255)

Target: CLDN14 (Claudin 14 (CLDN14))

Reactivity: Human, Monkey, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This CLDN14 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CLDN14 Antibody

Binding Specificity: AA 227-239

Specificity: Human CLDN14 / Claudin 14. Reported variants represent identical protein (NP_036262.1, NP_652763.1, NP_001139551.1, NP_001139550.1 and NP_001139549.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%) Mouse, Rat, Hamster, Elephant, Bovine, Horse, Opossum, Chicken, Lizard (92%) Panda, Dog, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-SATHSGYRLNDYV from the C-terminus of human CLDN14 / Claudin 14 (NP_036262.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Rabbit (100%), Mouse, Rat, Hamster, Elephant, Bovine, Horse, Opossum, Chicken, Lizard (92%), Panda, Dog, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for CLDN14

Target: CLDN14 (Claudin 14 (CLDN14))

Alternative Name: CLDN14 / Claudin 14

Background: Name/Gene ID: CLDN14
Family: Claudin

Synonyms: CLDN14, Claudin 14, DFNB29, Claudin-14

Gene ID: 23562

NCBI Accession: NP_036262

UniProt: O95500

Pathways: Cell-Cell Junction Organization, Hepatitis C