KPNB1 antibody (AA 851-863)

Catalog No. ABIN337256

The Goat Polyclonal anti-KPNB1 antibody is suitable to detect KPNB1 in samples from Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Bat, Monkey and Pig. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for KPNB1 antibody (AA 851-863) (ABIN337256)

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Bat, Monkey, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This KPNB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KPNB1 Antibody

Binding Specificity: AA 851-863

Specificity: Human KPNB1.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%) Opossum, Chicken, Lizard, Xenopus (92%) Stickleback, Pufferfish, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-RRSKTNKAKTLAT from the C-terminus of human KPNB1 (NP_002256.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig (100%), Opossum, Chicken, Lizard, Xenopus (92%), Stickleback, Pufferfish, Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:32000), WB (1:32000, 0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for KPNB1

Target: KPNB1 (Karyopherin (Importin) beta 1 (KPNB1))

Alternative Name: KPNB1 / Importin Beta

Background: Name/Gene ID: KPNB1

Synonyms: KPNB1, Importin 90, Importin Beta, Importin beta-1 subunit, IPO1, IPOB, NTF97, Impnb, Importin 1, Karyopherin subunit beta-1, Nuclear factor p97, PTAC97, IMB1, Importin subunit beta-1, Importin-90, Karyopherin (importin) beta 1

Gene ID: 3837

NCBI Accession: NP_002256

UniProt: Q14974

Pathways: Protein targeting to Nucleus