LEFTY2 antibody

Catalog No. ABIN337262

The Mouse Monoclonal anti-LEFTY2 antibody is suitable to detect LEFTY2 in samples from Human. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for LEFTY2 antibody (ABIN337262)

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LEFTY2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 7C5G1H6H10

Product Details anti-LEFTY2 Antibody

Specificity: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.

Purification: Protein A purified

Immunogen: Recombinant human LEFTY2.

Type of Immunogen: Recombinant protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (10 μg/mL), WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide. Sourced in TCS.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for LEFTY2

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Alternative Name: TGFB4 / LEFTY2

Background: Name/Gene ID: LEFTY2
Family: TGF beta

Synonyms: LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4

Gene ID: 7044

UniProt: O00292