Transgelin antibody (AA 190-201)

Catalog No. ABIN337285

This Goat Polyclonal antibody specifically detects Transgelin in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Cow, Pig, Dog, Horse, Hamster, Monkey, Bat and Xenopus laevis.

Quick Overview for Transgelin antibody (AA 190-201) (ABIN337285)

Target: Transgelin (TAGLN)

Reactivity: Human, Mouse, Rat, Cow, Pig, Dog, Horse, Hamster, Monkey, Bat, Xenopus laevis

Host: Goat

Clonality: Polyclonal

Conjugate: This Transgelin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Transgelin Antibody

Binding Specificity: AA 190-201

Specificity: Human TAGLN / SM22. NP_003177.2 and NP_001001522.1 represent identical protein.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Xenopus (100%) Stickleback (92%) Turkey, Chicken, Lizard, Catfish, Salmon, Pike, Zebrafish (83%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-MTGYGRPRQIIS from the C-terminus of human TAGLN / SM22 (NP_003177.2). Percent identity by BLAST analysis: Human, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bat, Bovine, Horse, Pig, Opossum, Xenopus (100%), Stickleback (92%), Turkey, Chicken, Lizard, Catfish, Salmon, Pike, Zebrafish (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:2000), WB (1:2000, 0 - 0 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Transgelin

Target: Transgelin (TAGLN)

Alternative Name: TAGLN / Transgelin / SM22

Background: Name/Gene ID: TAGLN

Synonyms: TAGLN, 22 kDa actin-binding protein, SMCC, TAGLN1, WS3-10, Transgelin, Transgelin variant 2, SM22, Protein WS3-10, SM22-alpha

Gene ID: 6876

NCBI Accession: NP_003177

UniProt: Q01995