TIMP3 antibody (AA 170-188)

Catalog No. ABIN337298

The Mouse Monoclonal anti-TIMP3 antibody has been validated for WB, IHC, IHC (p) and IHC (fro). It is suitable to detect TIMP3 in samples from Human, Cow, Dog, Horse, Pig, Sheep and Monkey.

Quick Overview for TIMP3 antibody (AA 170-188) (ABIN337298)

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Reactivity: Human, Cow, Dog, Horse, Pig, Sheep, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TIMP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-TIMP3 Antibody

Binding Specificity: AA 170-188

Brand: IHC-plus™

Specificity: Recognizes human TIMP-3. Does not react with human TIMP-1 and human TIMP-2. Species cross-reactivity: rabbit TIMP-3. Sequence homology with rat (18/19).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Sheep, Panda, Dog, Bovine, Horse, Pig (100%) Mouse, Rat, Hamster, Bat, Opossum, Turkey, Chicken, Lizard (95%) Elephant (89%) Xenopus (84%).

Purification: Purified

Immunogen: Oligopeptide corresponding to aa170-188( SWYRGWAPPDKSIINATDP) on human tissue inhibitor of metalloproteinases-3. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Baboon, Monkey, Galago, Marmoset, Sheep, Panda, Dog, Bovine, Horse, Pig (100%), Mouse, Rat, Hamster, Bat, Opossum, Turkey, Chicken, Lizard (95%), Elephant (89%), Xenopus (84%).

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-Fr, IHC-P (10 μg/mL), WB (2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.0, 2 % BSA (protease free).

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TIMP3

Target: TIMP3 (TIMP Metallopeptidase Inhibitor 3 (TIMP3))

Alternative Name: TIMP3

Background: Name/Gene ID: TIMP3

Synonyms: TIMP3, HSMRK222, K222TA2, MIG-5 protein, K222, SFD, Metalloproteinase inhibitor 3, Protein MIG-5, TIMP-3

Gene ID: 7078

UniProt: P35625