Complement Factor H antibody (AA 577-588)

Catalog No. ABIN337307

The Goat Polyclonal anti-Complement Factor H antibody is suitable to detect Complement Factor H in samples from Human. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for Complement Factor H antibody (AA 577-588) (ABIN337307)

Target: Complement Factor H (CFH)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Complement Factor H antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Complement Factor H Antibody

Binding Specificity: AA 577-588

Specificity: Human CFH / Factor H. This antibody is expected to recognize isoform a (NP_000177.2) only.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-HLVPDRKKDQYK from an internal region of human CFH / Factor H (NP_000177.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), Peptide-ELISA (1:32000), WB (0.03 - 0.1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Complement Factor H

Target: Complement Factor H (CFH)

Alternative Name: CFH / Complement Factor H

Background: Name/Gene ID: CFH

Synonyms: CFH, Adrenomedullin binding protein, AHUS1, AMBP1, ARMS1, ARMD4, Beta-1-H-globulin, Factor H, Factor H-like 1, HF, HF2, H factor 1, H factor 2 (complement), HUS, HF1, Beta-1H, CFHL3, Complement factor H, H factor 1 (complement)

Gene ID: 3075

NCBI Accession: NP_000177

UniProt: P08603

Pathways: Complement System, Cellular Response to Molecule of Bacterial Origin