TPI1 antibody (AA 274-286)

Catalog No. ABIN337332

The Goat Polyclonal anti-TPI1 antibody (ABIN337332) specifically detects TPI1 in WB, ELISA, IHC and IHC (p). The antibody is reactive with Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Bat, Hamster and Monkey samples.

Quick Overview for TPI1 antibody (AA 274-286) (ABIN337332)

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Reactivity: Human, Mouse, Rat, Rabbit, Dog, Cow, Horse, Bat, Hamster, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This TPI1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TPI1 Antibody

Binding Specificity: AA 274-286

Specificity: Human TPI1. This antibody is expected to recognise both reported isoforms.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Salmon, Pike (100%) Pig, Chicken, Xenopus, Smelt, Pufferfish, Drosophila (92%) Catfish, Stickleback, Zebrafish, Neurospora, Beetle, Sordaria, Tick, Chaetomium, Thielavia (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-LKPEFVDIINAKQ from the C-terminus of human TPI1 (NP_000356.1, NP_001152759.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Salmon, Pike (100%), Pig, Chicken, Xenopus, Smelt, Pufferfish, Drosophila (92%), Catfish, Stickleback, Zebrafish, Neurospora, Beetle, Sordaria, Tick, Chaetomium, Thielavia (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:16000), WB (0.01 - 0.03 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TPI1

Target: TPI1 (Triosephosphate Isomerase 1 (TPI1))

Alternative Name: TPI1 / TPI

Background: Name/Gene ID: TPI1

Synonyms: TPI1, Triose-phosphate isomerase, Triosephosphate isomerase, TPI, Triosephosphate isomerase 1, TIM

Gene ID: 7167

NCBI Accession: NP_000356, NP_001152759

UniProt: P60174

Pathways: Cell RedoxHomeostasis