MMP2 antibody (AA 468-483)

Catalog No. ABIN337360

This Mouse Monoclonal antibody specifically detects MMP2 in WB, IHC, ELISA, IHC (p) and IHC (fro). It exhibits reactivity toward Human and Pig.

Quick Overview for MMP2 antibody (AA 468-483) (ABIN337360)

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This MMP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-MMP2 Antibody

Binding Specificity: AA 468-483

Brand: IHC-plus™

Specificity: Recognizes the precursor and active forms of human MMP-2. Species cross-reactivity: mouse, rat, rabbit, porcine, bovine and equine MMP-2, but not with human MMP-1, 3, 9 and 13.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%) Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%) Bovine (88%) Chicken (81%).

Purification: Purified

Immunogen: Synthetic oligopeptide corresponding to aa residues 468-483 (VTPRDKPMGPLLVATF) of human MMP-2. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Pig (100%), Marmoset, Mouse, Rat, Sheep, Hamster, Elephant, Panda, Dog, Rabbit, Horse (94%), Bovine (88%), Chicken (81%).

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr (0.9 μg/mL), IHC-P (10 μg/mL), WB (1 - 5 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M PBS,  pH 7.0, 2 % BSA (protease free).

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for MMP2

Target: MMP2 (Matrix Metalloproteinase 2 (MMP2))

Alternative Name: MMP2 / MMP-2

Background: Name/Gene ID: MMP2
Subfamily: Metallopeptidase M10A
Family: Protease

Synonyms: MMP2, 72 kd type iv collagenase, 72 kDa gelatinase, 72 kd gelatinase, 72 kDa type IV collagenase, CLG4A, CLG4, GelA, Gelatinase A, Matrix metalloproteinase-2, MMP-2, MONA, Neutrophil gelatinase, TBE-1, Tbe-1 metalloprotease, Collagenase type IV-A, Matrix metalloproteinase-II, MMP-II

Gene ID: 4313

UniProt: P08253

Pathways: Activation of Innate immune Response