Iba1 antibody (AA 149-161)

Catalog No. ABIN337364

The Goat Polyclonal anti-Iba1 antibody is suitable to detect Iba1 in samples from Human, Rat, Mouse, Pig, Monkey and Hamster. It has been validated for WB, IHC, ELISA and IHC (p).

Quick Overview for Iba1 antibody (AA 149-161) (ABIN337364)

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Reactivity: Human, Rat, Mouse, Pig, Monkey, Hamster

Host: Goat

Clonality: Polyclonal

Conjugate: This Iba1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Iba1 Antibody

Binding Specificity: AA 149-161

Specificity: Human AIF1 / IBA1. This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Pig (100%) Goat, Elephant, Bovine, Horse, Rabbit, Platypus (92%) Panda, Dog, Bat (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-TGPPAKKAISELP from the C-terminus of human AIF1 / IBA1 (NP_116573.1, NP_001614.3). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Pig (100%), Goat, Elephant, Bovine, Horse, Rabbit, Platypus (92%), Panda, Dog, Bat (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:128000), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Iba1

Target: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Alternative Name: AIF1 / IBA1

Background: Name/Gene ID: AIF1

Synonyms: AIF1, AIF-1, Em:AF129756.17, G1, IRT-1, Protein G1, IBA1, IRT1

Gene ID: 199

NCBI Accession: NP_116573, NP_001614

UniProt: P55008

Pathways: Smooth Muscle Cell Migration