SMAD9 antibody (AA 166-179)
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- Target See all SMAD9 Antibodies
- SMAD9 (SMAD Family Member 9 (SMAD9))
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Binding Specificity
- AA 166-179
- Reactivity
- Human, Mouse, Rat, Dog, Bat, Chicken, Cow, Hamster, Horse, Monkey, Pig, Rabbit
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This SMAD9 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Brand
- IHC-plus™
- Specificity
- Human SMAD9. This antibody is not expected to cross-react with SMAD1 and SMAD5.
- Predicted Reactivity
- Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%) Opossum, Platypus, Lizard (93%) Stickleback (86%).
- Purification
- Immunoaffinity purified
- Immunogen
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Synthetic peptide C-HSEPLMPHNATYPD from an internal region of human SMAD9 (NP_005896.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Turkey, Chicken (100%), Opossum, Platypus, Lizard (93%), Stickleback (86%).
Type of Immunogen: Synthetic peptide - Top Product
- Discover our top product SMAD9 Primary Antibody
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- Application Notes
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Approved: IHC, IHC-P (5 μg/mL), Peptide-ELISA (1:64000), WB (0.3 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- Tris-buffered saline, pH 7.3, 0.5 % BSA, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- SMAD9 (SMAD Family Member 9 (SMAD9))
- Alternative Name
- SMAD9 (SMAD9 Products)
- Synonyms
- SMAD8 antibody, MADH6 antibody, MADH9 antibody, PPH2 antibody, SMAD8A antibody, SMAD8B antibody, Madh8 antibody, Madh9 antibody, Smad8 antibody, SMAD family member 9 antibody, SMAD9 antibody, smad9 antibody, Smad9 antibody
- Background
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Name/Gene ID: SMAD9
Synonyms: SMAD9, MAD homolog 9, SMAD 9, SMAD8A, SMAD8, Smad9, MADH9, SMAD8B, MADH6, Mothers against DPP homolog 9, SMAD family member 9 - Gene ID
- 4093
- NCBI Accession
- NP_005896
- UniProt
- O15198
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