PAR1 antibody (N-Term)

Catalog No. ABIN337370

The Mouse Monoclonal anti-PAR1 antibody has been validated for ELISA, IHC, FACS, IF and IHC (p). It is suitable to detect PAR1 in samples from Human.

Quick Overview for PAR1 antibody (N-Term) (ABIN337370)

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAR1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: ATAP2

Product Details anti-PAR1 Antibody

Binding Specificity: N-Term

Brand: IHC-plus™

Specificity: Recognizes the cleaved and intact forms of human thrombin receptor PAR1, N-terminus. Does not cross-react with PAR2.

Purification: Protein A purified

Immunogen: A synthetic peptide corresponding to the N-terminal region of the human thrombin receptor PAR1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), Flo (10 - 20 μg/mL), IF (1 - 3 μg/mL), IHC, IHC-P (10 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Immunofluorescence (Methanol or acetone fixed CHRF cells): 1-3 μg/mL. Flow Cytometry (Methanol or acetone fixed CHRF cells): 10-20 μg/mL. ELISA: 0.1-1.0 μg/mL.

Not recommended for: WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PAR1

Target: PAR1 (F2R) (Coagulation Factor II (thrombin) Receptor (F2R))

Alternative Name: F2R / Thrombin Receptor / PAR1

Background: Name/Gene ID: F2R
Subfamily: Proteinase-activated
Family: GPCR

Synonyms: F2R, CF2R, Coagulation factor II receptor, PAR1, PAR-1, Thrombin receptor, TR, HTR, Protease-activated receptor 1

Gene ID: 2149

UniProt: P25116

Pathways: Nuclear Receptor Transcription Pathway, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus