Caspase 6 antibody (Cleavage Site)

Catalog No. ABIN337396

This Rabbit Polyclonal antibody specifically detects Caspase 6 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rabbit.

Quick Overview for Caspase 6 antibody (Cleavage Site) (ABIN337396)

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Reactivity: Human, Mouse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 6 Antibody

Binding Specificity: Cleavage Site

Brand: IHC-plus™

Specificity: Caspase-6 Antibody recognizes endogenous levels of both full length caspase-6 (35kD) and the small subunit of caspase-6 resulting from cleavage at Asp194 (15kD). This antibody does not recognize other caspases. Recognizes human, mouse, rabbit.

Purification: Protein A purified

Immunogen: Synthetic peptide KLH coupled corresponding to residues surrounding the cleavage site of caspase-6.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (5 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Caspase 6

Target: Caspase 6 (CASP6) (Caspase 6, Apoptosis-Related Cysteine Peptidase (CASP6))

Alternative Name: CASP6 / Caspase 6

Background: Name/Gene ID: CASP6
Subfamily: Cysteine C14
Family: Protease

Synonyms: CASP6, Apoptotic protease Mch-2, Caspase 6, CASP-6, Caspase-6, Mch-2, MCH2

Gene ID: 839

UniProt: P55212

Pathways: Apoptosis, Caspase Cascade in Apoptosis