HSF1 antibody

Catalog No. ABIN337399

This Rabbit Polyclonal antibody specifically detects HSF1 in WB, IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Rat, Rabbit and Monkey.

Quick Overview for HSF1 antibody (ABIN337399)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-HSF1 Antibody

Brand: IHC-plus™

Specificity: Recognizes human HSF-1 at ~85kD protein in unstressed cells and an ~95kD protein in heat shocked cells, corresponding to the molecular masses of the inactive and activated forms respectively of HSF-1. Species cross-reactivity: mouse, rat, monkey, rabbit.

Purification: Protein A purified

Immunogen: Recombinant human HSF-1 expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (1:500)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Positive control: Recombinant Human HSF-1 Protein.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: Name/Gene ID: HSF1

Synonyms: HSF1, HSTF 1, Heat shock factor protein 1, HSF 1, HSTF1

Gene ID: 3297

UniProt: Q00613