Nitrotyrosine antibody

Catalog No. ABIN339840

This anti-Nitrotyrosine antibody is a Rabbit Polyclonal antibody detecting Nitrotyrosine in WB, ELISA and ICC. Suitable for .

Quick Overview for Nitrotyrosine antibody (ABIN339840)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Product Details anti-Nitrotyrosine Antibody

Specificity: Detects proteins and peptides containing nitro-tyrosine in a manner independent of the surrounding amino acid sequence. It is a valuable tool for identifying new nitrated proteins as well as for assaying protein nitration and measuring levels of nitrated proteins in tissues and samples. Does not cross-react with unmodified tyrosine or with phospho-tyrosine.

Purification: Protein A purified

Immunogen: Synthetic nitro-tyrosine containing peptides coupled to KLH.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:1000), ICC, WB (1:1000)

Usage: Suitable for use in ELISA, Western Blot and Immunocytochemistry (ABC detection method). Western Blot: 1:1000, incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking, overnight. Immunocytochemistry (ABC): 1:50. ELISA: 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical