Phosphothreonine antibody

Catalog No. ABIN340103

The Rabbit Polyclonal anti-Phosphothreonine antibody has been validated for WB, ELISA and IP. It is suitable to detect Phosphothreonine in samples from .

Quick Overview for Phosphothreonine antibody (ABIN340103)

Target: Phosphothreonine

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-Phosphothreonine Antibody

Specificity: Recognizes phosphothreonine, peptidyl-phosphothreonine and threonine-phosphorylated proteins. Species cross-reactivity: species independent.

Purification: Immunoaffinity purified

Immunogen: Phosphothreonine conjugated to KLH and phosvitin.

Type of Immunogen: Hapten

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)

Usage: Suitable for use in Western Blot, ELISA, and Immunoprecipitation. Western Blot: 2 μg/mL, Mouse brain lysate. Incubate at RT for 4 hrs. ELISA: 0.5 μg/mL (kinase assay). Binding of Ly-pT-Gly-HRP. (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7, 0.01 % sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Phosphothreonine

Target: Phosphothreonine

Target Type: Amino Acid