Phosphothreonine antibody

Catalog No. ABIN340104

The Mouse Monoclonal anti-Phosphothreonine antibody (ABIN340104) specifically detects Phosphothreonine in WB, IP and ELISA.

Quick Overview for Phosphothreonine antibody (ABIN340104)

Target: Phosphothreonine

Host: Mouse

Clonality: Monoclonal

Conjugate: This Phosphothreonine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), ELISA

Product Details anti-Phosphothreonine Antibody

Specificity: Recognizes Phosphothreonine, peptidyl-phosphothreonine, and threonine-phosphorylated proteins. Binding of Ly-pT-Gly-HRP (0.2 μg/100 μL/well) to an anti-phosphothreonine coated microplate (0.5 μg/100 μL/well) is inhibited by 95 % with the addition of 2-10 μg/mL anti-phosphothreonine. No inhibition is evident by inhibition of phosphoserine, phosphotyrosine or threonine at concentrations of up to 1 mg/mL. Species cross-reactivity: species independent.

Purification: Immunoaffinity purified

Immunogen: Keyhole Limpet Hemocyanin (KLH)- conjugated phosphothreonine conjugates and Phosvitin.

Type of Immunogen: Purified protein

Isotype: IgG2a kappa

Application Details

Application Notes: Approved: ELISA (0.5 μg/mL), IP, WB (2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, and Immunoprecipitation. ELISA: 0.5 μg/mL (kinase assay). Western Blot: 2 μg/mL versus mouse brain lysate. Incubate at RT for 4 hours.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Phosphothreonine

Target: Phosphothreonine

Target Type: Amino Acid