BRK1 antibody (C-Term)

Catalog No. ABIN341474

The Rabbit Polyclonal anti-BRK1 antibody is suitable to detect BRK1 in samples from Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Horse, Pig, Monkey and Xenopus laevis. It has been validated for IHC, WB and IHC (p).

Quick Overview for BRK1 antibody (C-Term) (ABIN341474)

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Horse, Pig, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRK1 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BRK1 Antibody

Binding Specificity: C-Term

Specificity: Human C3orf10

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Xenopus, Pike, Catfish, Salmon, Sablefish, Zebrafish (100%) Tick (92%) Drosophila, Mosquito (85%).

Purification: Protein A purified

Immunogen: Synthetic peptide from C-Terminus of human BRK1 (Q8WUW1, NP_060932). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Horse, Pig, Opossum, Xenopus, Seabass, Catfish, Salmon, Sablefish, Stickleback, Pike, Zebrafish (100%), Drosophila (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P, WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 2.5 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRK1

Target: BRK1 (BRICK1, SCAR/WAVE Actin-Nucleating Complex Subunit (BRK1))

Alternative Name: C3orf10

Background: Name/Gene ID: BRK1

Synonyms: BRK1, C3orf10, MDS027, HHBrk1, Probable protein BRICK1, Protein BRICK1, HSPC300

Gene ID: 55845

NCBI Accession: NP_060932

UniProt: Q8WUW1

Pathways: RTK Signaling